141808-99-3Relevant academic research and scientific papers
A lipid-lipase aggregate with ether linkage as a new type of immobilized enzyme for enantio-selective hydrolysis in organic solvents
Akita,Umezawa,Matsukura,Oishi
, p. 318 - 324 (2007/10/02)
For the purpose of carrying out smoothly enzymatic reaction of water-insoluble substrates in organic solvents, a new type of immobilized enzyme, a lipid-lipase aggregate, was developed. In order to prepare various kinds of lipid-lipase aggregates, 27 kinds of dialkyl ether-type phospholipid analogues were newly synthesized and used for the preparation of aggregates with lipase. Thus obtained lipid-lipase aggregates were found to catalyze the enantioselective hydrolysis of the (+)-α-acyloxy ester 2 much more efficiently than lipase immobilized with synthetic prepolymer (ENTP-4000) in water-saturated isopropyl ether. Namely, the reaction time became much shorter (2 to 3 d for completion as compared with 21 d) and the chemical and optical yields of the reaction products were found to be high.
