1421322-31-7Relevant academic research and scientific papers
Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide- modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids
Goubet, Astrid,Chardon, Antoine,Kumar, Pawan,Sharma, Pawan K.,Veedu, Rakesh N.
, p. 761 - 763 (2013)
C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.
