142439-92-7 Usage
Description
Biotin-bisamido-SS-NHS is a cleavable reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. The cross-bridge and extended spacer arm are helpful in minimizing steric hindrance involved with the binding to avidin or streptavidin probes. This biotin compound is membrane-permeable and useful for intracellular labeling. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP to remove the biotin label.
Uses
NHS SS Biotin is a amine reactive biotinylation reagent that labels antibodies and other proteins and molecules that contain primary amines. NHS SS Biotin reacts efficiently with pirmary amino groups
in alkaline buffers to form stable amide bonds. The disulfide bond in the spacer arm of NHS SS Biotin is also cleavable using reducing agents such as TCEP and DTT.
Check Digit Verification of cas no
The CAS Registry Mumber 142439-92-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,2,4,3 and 9 respectively; the second part has 2 digits, 9 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 142439-92:
(8*1)+(7*4)+(6*2)+(5*4)+(4*3)+(3*9)+(2*9)+(1*2)=127
127 % 10 = 7
So 142439-92-7 is a valid CAS Registry Number.
142439-92-7Relevant articles and documents
Disulfide-bond-containing long arm biotin and preparation method thereof
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Paragraph 0016; 0017, (2017/12/27)
The invention provides disulfide-bond-containing long arm biotin and a preparation method thereof. The compound can be applied to the bio-detecting field, reduces influences of the steric effect, and improves sensitivity of detection. The structure formula of the disulfide-bond-containing long arm biotin is shown in the description; biotin is taken as a raw material, and is subjected to acylation and active ester synthesis through acylation ethanediamine and thiodipropionic acid to prepare the disulfide-bond-containing biotin.