1427084-77-2Relevant academic research and scientific papers
Side-chain protected oligopeptide fragment condensation using subtilisins in organic solvents
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Page/Page column 35; 36, (2017/04/11)
Method for enzymatically synthesizing an oligopeptide, comprising the coupling of an (optionally N-protected) protected oligopeptide ester with an (optionally C-protected) protected oligopeptide nucleophile in an organic solvent or an organic solvent mixture having a water content of 0.1 vol % or less, by a subtilisin in any possible form.
Enzymatic fragment condensation of side chain-protected peptides using subtilisin A in anhydrous organic solvents: A general strategy for industrial peptide synthesis
Nuijens, Timo,Schepers, Annette H. M.,Cusan, Claudia,Kruijtzer, John A. W.,Rijkers, Dirk T. S.,Liskamp, Rob M. J.,Quaedflieg, Peter J. L. M.
, p. 287 - 293 (2013/05/21)
Herein, the enzymatic condensation of side chain-protected peptide fragments using subtilisin A in anhydrous organic solvents is described. A screening with dipeptide Cbz-Val-Xxx carboxamidomethyl esters with H-Xxx-Val-NH2 nucleophiles was performed, wherein Xxx stands for every (side chain-protected) amino acid residue. Finally, it was demonstrated that it is feasible to enzymatically condense larger peptide fragments (up to the 10-mer level) bearing multiple side chain-protecting groups with very high conversion.
