1428554-87-3Relevant academic research and scientific papers
Alkyne-tag Raman imaging for visualization of mobile small molecules in live cells
Yamakoshi, Hiroyuki,Dodo, Kosuke,Palonpon, Almar,Ando, Jun,Fujita, Katsumasa,Kawata, Satoshi,Sodeoka, Mikiko
, p. 20681 - 20689 (2013/02/23)
Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous molecule in live cells. Here, we show that alkyne-tag Raman imaging (ATRI) is a promising approach for visualizing nonimmobilized small molecules in live cells. An examination of structure-Raman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogues in live cells. Cellular concentrations of diyne-tagged CoQ analogues could be semiquantitatively estimated. Finally, simultaneous imaging of two small molecules, 5-ethynyl-2′-deoxyuridine (EdU) and a CoQ analogue, with distinct Raman tags was demonstrated.
