143571-70-4Relevant academic research and scientific papers
Preparation of base-deuterated 2′-deoxyadenosine nucleosides and their site-specific incorporation into DNA
Chirakul,Litzer,Sigurdsson
, p. 1903 - 1913 (2001)
We report a four-step synthesis of 2′-deoxy-2-deuteroadenosine from 2′-deoxyadenosine in 38% overall yield. The more accessible 2′-deoxy-8-deuteroadenosine was also prepared and incorporated into DNA by automated solid phase synthesis (80% deuterium) using N6-benzoyl-2′-deoxy-8-deuteroadenosine-3′-O- (2-cyanoethyl-N,N-diisopropylphos-phoramidite) in combination with acetyl-protected deoxycytidine and phenoxyacetyl-protected purine phosphoramidites.
