1436382-65-8Relevant articles and documents
A substrate for the detection of broad specificity α-l- arabinofuranosidases with indirect release of a chromogenic group
Borsenberger, Vinciane,Dornez, Emmie,Desrousseaux, Marie-Laure,Courtin, Christophe M.,O'Donohue, Michael J.,Fauré, Régis
, p. 3063 - 3066 (2013/07/04)
The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal α-l-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several α-l-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to α-l- arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties.