1447606-76-9Relevant academic research and scientific papers
Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
Vengut-Climent, Empar,Terrazas, Montserrat,Lucas, Ricardo,Arévalo-Ruiz, Matilde,Eritja, Ramón,Morales, Juan Carlos
, p. 4048 - 4051 (2013)
Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.
