1448012-76-7Relevant academic research and scientific papers
Impact of donor binding on polymerization catalyzed by KfoC by regulating the affinity of enzyme for acceptor
Xue, Jiajun,Jin, Lan,Zhang, Xinke,Wang, Fengshan,Ling, Peixue,Sheng, Juzheng
, p. 844 - 855 (2016)
Background Currently marketed chondroitin sulfate isolated from animal sources and structurally quite heterogeneous. Synthesis of structurally defined chondroitin sulfate is highly desired. The capsular polysaccharide from Escherichia coli strain K4 is si
Toward the chemoenzymatic synthesis of heparan sulfate oligosaccharides: Oxidative cleavage of p-nitrophenyl group with ceric ammonium salts
Cai, Chao,Li, Lingyun,Harvey, Cate,Liu, Jian,Linhardt, Robert J.
, p. 4471 - 4474 (2013)
We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly simplifies oligosaccharide purification on C-18 resin. Employing ceric ammonium nitrate as oxidative reagent to remove the p-NP group unexpectedly also removed the glucuronic acid residue at the reducing-end, affording a smaller oligosaccharide. The application of ceric ammonium sulfate allowed the removal of the p-NP without concomitant loss of the adjacent glucuronic acid offering a route to longer heparin sulfate oligosaccharide products.
Uncovering the catalytic direction of chondroitin ac exolyase-from the reducing end towards the non-reducing end
Yin, Feng-Xin,Wang, Feng-Shan,Sheng, XJu-Zheng
, p. 4399 - 4406 (2016/05/09)
Glycosaminoglycans (GAGs) are polysaccharides that play vital functional roles in numerous biological processes, and compounds belonging to this class have been implicated in a wide variety of diseases. Chondroitin AC lyase (ChnAC) (EC 4.2.2.5) catalyzes
