1448148-07-9Relevant academic research and scientific papers
Synthesis of multivalent carbohydrate-centered glycoclusters as nanomolar ligands of the bacterial lectin LecA from pseudomonas aeruginosa
Gening, Marina L.,Titov, Denis V.,Cecioni, Samy,Audfray, Aymeric,Gerbst, Alexey G.,Tsvetkov, Yury E.,Krylov, Vadim B.,Imberty, Anne,Nifantiev, Nikolay E.,Vidal, Sebastien
, p. 9272 - 9285 (2013)
A family of fifteen glycoclusters based on a cyclic oligo-(1→6)- β-D-glucosamine core has been designed as potential inhibitors of the bacterial lectin LecA with various valencies (from 2 to 4) and linkers. Evaluation of their binding properties towards LecA has been performed by a combination of hemagglutination inhibition assays (HIA), enzyme-linked lectin assays (ELLA), and isothermal titration microcalorimetry (ITC). Divalent ligands displayed dissociation constants in the sub-micromolar range and tetravalent ligands displayed low nanomolar affinities for this lectin. The influence of the linker could also be demonstrated; aromatic moieties are the best scaffolds for binding to the lectin. Copyright
