145224-94-8 Usage
Description
MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.
Chemical Properties
white crystalline powder
Uses
Different sources of media describe the Uses of 145224-94-8 differently. You can refer to the following data:
1. MES Monohydrate has been used:To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assayAs a buffering agent to stabilize enzymatic solutionsAs a component of PAGE running buffer
2. MES monohydrate is a biological buffer often referred to as Good?s buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.
3. MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
Application
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.MES Monohydrate has been used:To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.As a buffering agent to stabilize enzymatic solutions.As a component of PAGE running buffer.
General Description
buffer substance MES
Preparation and handling
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Precautions
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
Check Digit Verification of cas no
The CAS Registry Mumber 145224-94-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,5,2,2 and 4 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 145224-94:
(8*1)+(7*4)+(6*5)+(5*2)+(4*2)+(3*4)+(2*9)+(1*4)=118
118 % 10 = 8
So 145224-94-8 is a valid CAS Registry Number.
