146986-50-7

Basic information

• Product Name: Y-27632 DIHYDROCHLORIDE
• Synonyms: ROCK INHIBITOR;(R)-(+)-TRANS-N-(4-PYRIDYL)-4-(1-AMINOETHYL)-CYCLOHEXANECARBOXAMIDE 2HCL;(R)-(+)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL)CYCLOHEXANECARBOXAMIDE DIHYDROCHLORIDE;(+)-R-TRANS-4-(AMINOETHYL)-N-(4-PYRIDYL)-CYCLOHEXANECARBOXAMIDE 2HCL H2O;Y-27632;Y-27632 DIHYDROCHLORIDE;TRANS-4-[(1R)-1-AMINOETHYL]-N-4-PYRIDINYLCYCLOHEXANECARBOXAMIDE DIHYDROCHLORIDE;Y-276322HCl
• CAS NO: 146986-50-7
• Molecular Formula: C14H23Cl2N3O
• Molecular Weight: 320.26
• EINECS: 1312995-182-4
• Product Categories: Protein Kinase;Signalling;Inhibitor;Inhibitors
• Mol File: 146986-50-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 462.595 °C at 760 mmHg
• Flash Point: 233.569 °C
• Appearance: white/solid
• Density: 1.137 g/cm³
• Storage Temp.: 2-8°C
• Solubility: H2O: 14 mg/mL
• PKA: 13.53±0.40(Predicted)
• Stability: Hygroscopic
• CAS DataBase Reference: Y-27632 DIHYDROCHLORIDE(CAS DataBase Reference)
• NIST Chemistry Reference: Y-27632 DIHYDROCHLORIDE(146986-50-7)
• EPA Substance Registry System: Y-27632 DIHYDROCHLORIDE(146986-50-7)

Safety Data

• Hazard Codes: Xn
• Statements: 20/21/22
• Safety Statements: 36
• WGK Germany: 3
• Hazardous Substances Data: 146986-50-7(Hazardous Substances Data)

Usage

Description

Y-27632 DIHYDROCHLORIDE is a salt analog of Y-27632, which is a Rho-kinase inhibitor. It is a monocarboxylic acid amide with the chemical structure trans-[(1R)-1-aminoethyl]cyclohexanecarboxamide, where one of the nitrogens of the aminocarbonyl group is substituted by a pyridine nucleus. Y-27632 DIHYDROCHLORIDE has been demonstrated to exhibit inhibitory activity against the Rho-associated protein kinase (ROCK) enzyme.

Uses

Used in Pharmaceutical Industry:
Y-27632 DIHYDROCHLORIDE is used as a Rho-kinase inhibitor for various applications in the pharmaceutical industry. It plays a crucial role in modulating the activity of the ROCK enzyme, which is involved in numerous cellular processes, including cell proliferation, migration, and contraction. By inhibiting ROCK, Y-27632 DIHYDROCHLORIDE can potentially be used in the development of therapeutic strategies for various diseases and conditions.
Used in Research Applications:
In the field of research, Y-27632 DIHYDROCHLORIDE is used as a valuable tool for studying the role of Rho-kinase in cellular processes and signaling pathways. It can help researchers understand the molecular mechanisms underlying various diseases and identify potential targets for drug development.
Used in Cell Culture and Tissue Engineering:
Y-27632 DIHYDROCHLORIDE is used as a reagent in cell culture and tissue engineering applications. It can help promote cell adhesion, migration, and proliferation, which are essential for tissue repair and regeneration. By modulating the activity of Rho-kinase, Y-27632 DIHYDROCHLORIDE can improve the efficiency of tissue engineering strategies and contribute to the development of advanced biomaterials and scaffolds for tissue repair.
Used in Smooth Muscle Relaxation:
Y-27632 DIHYDROCHLORIDE is used as a smooth muscle relaxant in various applications, particularly in the treatment of conditions involving smooth muscle contraction, such as asthma, hypertension, and gastrointestinal disorders. By inhibiting Rho-kinase, Y-27632 DIHYDROCHLORIDE can help reduce smooth muscle tone and alleviate symptoms associated with these conditions.

Biological Activity

Selective inhibitor of the Rho-associated protein kinase p160ROCK. K i values are 0.14, 26, 25 and >250 μ M for p160ROCK, PKC, cAMP-dependent protein kinase and myosin light-chain kinase respectively. Also inhibits the protein kinase C-related protein kinase, PRK2 (IC 50 = 600nM). Smooth muscle relaxant and orally active in vivo . Increases survival rate of human embryonic stem (hES) cells undergoing cryopreservation.

references

[1] ishizaki t1, uehata m, tamechika i, keel j, nonomura k, maekawa m, narumiya s. pharmacological properties of y-27632, a specific inhibitor of rho-associated kinases. mol pharmacol. 2000 may;57(5):976-83.