148441-45-6Relevant articles and documents
Preparation of D-Glucose 6-Phosphate from Methanol and D-Ribose 5-Phosphate with Methylotrophic Enzymes
Yanase, Hideshi,Sato, Yusuke,Kita, Keiko,Sato, Yoshiyuki,Kato, Nobuo
, p. 308 - 312 (2007/10/02)
Formaldehyde was selectively incorporated into the C-1 position of D-fructose 6-phosphate by condensation with D-ribulose 5-phosphate catalyzed by a partially purified enzyme system for formaldehyde fixation in Methylomonas aminofaciens 77a.Much of the D-fructose 6-phosphate produced in this reaction was converted to D-glucose 6-phosphate by the addition of glucose-6-phosphate isomerase.A fed-batch reaction with periodic additions of the substrates afforded 56.2 g/liter D-glucose 6-phosphate and 26.8 g/liter D-fructose 6-phosphate.When methanol was used as the C1-donor, the yield of D-glucose 6-phosphate was high when alcohol oxidase was added.The optimum conditions for sugar phosphate production in the fed-batch reaction gave 45.6 g/liter D-glucose 6-phosphate and 16.4 g/liter D-fructose 6-phosphate in 165 min.The molar yield of the total sugar phosphates to methanol added was 95percent.The addition of H2O2 and catalase to the reaction system supplied molecular oxygen for methanol oxidation to formaldehyde by alcohol oxidase.