148504-34-1

Basic information

• Product Name: CALCEIN-AM
• Synonyms: 3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution;Calcein AM solution;4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester;Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester;Calcein AcetoxyMethyl Ester;N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester;NSC 689290;CALCEIN-ACETOXYMETHYL ESTER, DIACETATE
• CAS NO: 148504-34-1
• Molecular Formula: C46H46N2O23
• Molecular Weight: 994.85704
• EINECS: 200-664-3
• Product Categories: CCalcium Signaling Research Tools;Calcium Probes;Fluorescent;Stains and Dyes;Stains&Dyes, A to;Amines;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Xanthene
• Mol File: 148504-34-1.mol
• Article Data: 0

Chemical Properties

• Melting Point: >250℃
• Boiling Point: 982.7°Cat760mmHg
• Flash Point: 85 °C
• Appearance: Colorless/powder
• Density: 1.49g/cm³
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: n20/D 1.479
• Storage Temp.: −20°C
• Solubility: DMSO: soluble10mg/mL, clear, colorless to slightly yellow
• PKA: 2.66±0.50(Predicted)
• CAS DataBase Reference: CALCEIN-AM(CAS DataBase Reference)
• NIST Chemistry Reference: CALCEIN-AM(148504-34-1)
• EPA Substance Registry System: CALCEIN-AM(148504-34-1)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• RIDADR: NA 1993 / PGIII
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 148504-34-1(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 148504-34-1 differently. You can refer to the following data:
1. Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.
2. Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.
3. A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

General Description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

InChI:InChI=1/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3