149231-65-2 Usage
Description
AC-YVAD-AMC (N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin) is a sensitive fluorogenic substrate used in scientific research, specifically for studying enzymes. It is predominantly utilized to measure caspase-1 activity, an enzyme associated with programmed cell death. Moreover, the chemical can serve as an important tool in biochemical assays aimed at investigating processes such as apoptosis (cell death) and inflammation. When cleaved by the Caspase-1 enzyme, AC-YVAD-AMC exhibits fluorescence, which facilitates the monitoring and detection of the enzyme's activity. It is important to handle this chemical with care due to its sensitive nature.
Uses
Used in Biochemical Assays:
AC-YVAD-AMC is used as a sensitive fluorogenic substrate for measuring caspase-1 activity, which is crucial in understanding programmed cell death, apoptosis, and inflammation.
Used in Scientific Research:
AC-YVAD-AMC is used as a research tool for investigating the role of caspase-1 in various biological processes, including cell death and inflammation. Its fluorescence upon cleavage by caspase-1 allows for the easy monitoring and detection of enzyme activity.
Used in Drug Development:
AC-YVAD-AMC can be used as a screening tool in the development of new drugs targeting caspase-1, helping to identify potential therapeutic agents that can modulate the enzyme's activity and potentially treat diseases associated with apoptosis and inflammation.
Check Digit Verification of cas no
The CAS Registry Mumber 149231-65-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,9,2,3 and 1 respectively; the second part has 2 digits, 6 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 149231-65:
(8*1)+(7*4)+(6*9)+(5*2)+(4*3)+(3*1)+(2*6)+(1*5)=132
132 % 10 = 2
So 149231-65-2 is a valid CAS Registry Number.
149231-65-2Relevant articles and documents
A substrate combinatorial array for caspases
Lee, Dennis,Adams, Jerry L.,Brandt, Martin,DeWolf Jr., Walter E.,Keller, Paul M.,Levy, Mark A.
, p. 1667 - 1672 (2007/10/03)
An efficient strategy for the synthesis of a tetrapeptidyl substrate combinatorial array directed toward the caspases is described. Testing of this array with caspases 1 and 4 gave substrate hydrolytic profiles characteristic of each caspase, and permitted the identification of efficiently processed substrates. A comparison of this approach to that using a positional scanning library is presented.