149653-55-4Relevant academic research and scientific papers
A novel thymidine phosphoramidite synthon for incorporation of internucleoside phosphate linkers during automated oligodeoxynucleotide synthesis
Tabatadze, David,Zamecnik, Paul,Yanachkov, Ivan,Wright, George,Pierson, Katherine,Zhang, Surong,Bogdanov Jr., Alexei,Metelev, Valeri
, p. 157 - 172 (2008)
A novel thymidine phosphoramidite synthon was synthesized and successfully used for incorporation of primary amino groups, attached through a triethylene glycol linker to the internucleoside phosphates, at desired locations during automated oligodeoxynucleotide synthesis. The synthesized amino-linker bearing oligonucleotides are stable under deprotection conditions and exhibit Watson-Crick base-pairing properties. Covalent labeling of oligonucleotides with carbocyanine near-infrared fluorochromes resulted in 2.5 times higher labeling yields when compared with oligonucleotides containing base-attached aminolinkers. We anticipate that the developed synthetic approach will be useful for nucleotide sequence-specific attachment of single or multiple ligands or reporter molecules. Copyright Taylor & Francis Group, LLC.
