149695-85-2 Usage
Description
(Z-Ala-Ala-Ala-Ala)2Rh110 is a sensitive fluorogenic elastase substrate. The colorless and nonfluorescent (Z-Ala-Ala-Ala-Ala)2Rh110 is selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm.
in vitro
(z-ala-ala-ala-ala)2rh110 has been identified as a very sensitive fluorogenic elastase substrate. the colorless and nonfluorescent z-ala-ala-ala-ala)2rh110 was found to be selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which exhibited excellent spectral properties matching the optimal detection window of most fluorescence instruments. after cleavage, abs(max) is at 497 nm and em(max) is at 520 nm for rhodamine 110 [1].
references
[1] a. f. johnson, m. d. struthers, k. b. pierson, et al. nonisotopic dna detection system employing elastase and a fluorogenic rhodamine substrate. analytical chemistry 65, 2352-2359 (1993).
Check Digit Verification of cas no
The CAS Registry Mumber 149695-85-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,9,6,9 and 5 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 149695-85:
(8*1)+(7*4)+(6*9)+(5*6)+(4*9)+(3*5)+(2*8)+(1*5)=192
192 % 10 = 2
So 149695-85-2 is a valid CAS Registry Number.
InChI:InChI=1/C60H66N10O15/c1-31(55(77)70-56(78)36(6)66-51(73)34(4)64-53(75)38(8)68-59(81)83-30-40-19-13-10-14-20-40)61-41-23-25-45-47(27-41)84-48-28-42(24-26-46(48)60(45)44-22-16-15-21-43(44)57(79)85-60)69-54(76)35(5)65-50(72)32(2)62-49(71)33(3)63-52(74)37(7)67-58(80)82-29-39-17-11-9-12-18-39/h9-28,31-38,61H,29-30H2,1-8H3,(H,62,71)(H,63,74)(H,64,75)(H,65,72)(H,66,73)(H,67,80)(H,68,81)(H,69,76)(H,70,77,78)/t31-,32-,33-,34-,35-,36-,37-,38-,60?/m0/s1
149695-85-2Relevant articles and documents
Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate
Johnson, Arthur F.,Struthers, Mary D.,Pierson, Keith B.,Mangel, Walter F.,Smith, Lloyd M.
, p. 2352 - 2359 (2007/10/02)
An alternative fluorescence-based method has been developed for the direct detection of small quantities of DNA in solution. In this system, a serine protease (elastase) is coupled to a DNA oligonucleotide through a disulfide linkage. A bis-(tetraalanine)
