149695-85-2 Usage
Uses
Used in Research and Diagnostic Applications:
Bis(N-benzyloxycarbonyltetraalanyl)rhodamine is used as a research and diagnostic tool for monitoring elastase activity. Its selective cleavage by elastase and the resulting fluorescence make it an ideal indicator for studying the function and regulation of elastase in various biological processes.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, bis(N-benzyloxycarbonyltetraalanyl)rhodamine can be employed as a substrate for the development and testing of elastase inhibitors. These inhibitors may have potential therapeutic applications in treating conditions associated with elastase overactivity, such as certain types of cancer, inflammatory diseases, and tissue remodeling disorders.
Used in Drug Delivery Systems:
Similar to gallotannin, bis(N-benzyloxycarbonyltetraalanyl)rhodamine can be utilized in the development of novel drug delivery systems. By incorporating this substrate into drug carriers, researchers can potentially enhance the delivery, bioavailability, and therapeutic outcomes of elastase inhibitors or other related drugs.
in vitro
(z-ala-ala-ala-ala)2rh110 has been identified as a very sensitive fluorogenic elastase substrate. the colorless and nonfluorescent z-ala-ala-ala-ala)2rh110 was found to be selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which exhibited excellent spectral properties matching the optimal detection window of most fluorescence instruments. after cleavage, abs(max) is at 497 nm and em(max) is at 520 nm for rhodamine 110 [1].
references
[1] a. f. johnson, m. d. struthers, k. b. pierson, et al. nonisotopic dna detection system employing elastase and a fluorogenic rhodamine substrate. analytical chemistry 65, 2352-2359 (1993).
Check Digit Verification of cas no
The CAS Registry Mumber 149695-85-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,9,6,9 and 5 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 149695-85:
(8*1)+(7*4)+(6*9)+(5*6)+(4*9)+(3*5)+(2*8)+(1*5)=192
192 % 10 = 2
So 149695-85-2 is a valid CAS Registry Number.
InChI:InChI=1/C60H66N10O15/c1-31(55(77)70-56(78)36(6)66-51(73)34(4)64-53(75)38(8)68-59(81)83-30-40-19-13-10-14-20-40)61-41-23-25-45-47(27-41)84-48-28-42(24-26-46(48)60(45)44-22-16-15-21-43(44)57(79)85-60)69-54(76)35(5)65-50(72)32(2)62-49(71)33(3)63-52(74)37(7)67-58(80)82-29-39-17-11-9-12-18-39/h9-28,31-38,61H,29-30H2,1-8H3,(H,62,71)(H,63,74)(H,64,75)(H,65,72)(H,66,73)(H,67,80)(H,68,81)(H,69,76)(H,70,77,78)/t31-,32-,33-,34-,35-,36-,37-,38-,60?/m0/s1
149695-85-2Relevant academic research and scientific papers
Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate
Johnson, Arthur F.,Struthers, Mary D.,Pierson, Keith B.,Mangel, Walter F.,Smith, Lloyd M.
, p. 2352 - 2359 (2007/10/02)
An alternative fluorescence-based method has been developed for the direct detection of small quantities of DNA in solution. In this system, a serine protease (elastase) is coupled to a DNA oligonucleotide through a disulfide linkage. A bis-(tetraalanine)
