149989-48-0Relevant academic research and scientific papers
A base-labile group for 2′-OH protection of ribonucleosides: A major challenge for RNA synthesis
Lavergne, Thomas,Bertrand, Jean-Remi,Vasseur, Jean-Jacques,Debart, Francoise
, p. 9135 - 9138 (2008)
A base-labile group for 2'-OH protection of ribonucleosides was investigated. The solid support was dried by blowing argon through a DNA synthesizer and was first treated with 10% anhydrous piperidine in CH 3CN at room temperature for 15 minutes to eliminate cyanoethyl groups from phosphates. The piperidine solution was removed from the column and the solid support was washed with CH3CN. The three ammoniacal eluates were collected in a screw-capped glass vial and were left at room temperature for a further 1.5 hours to completely deprotect nucleobases and 2'-hydroxyl groups. The fully deprotected oligonucleotide was transferred to a 50 mL round-bottomed flask and isopropylamine was added to the solution before evaporation to dryness. It was observed that PivOM method provides highly pure RNA without any additional desalting step.
