1520083-35-5Relevant academic research and scientific papers
Efficient, traceless semi-synthesis of α-synuclein labeled with a fluoro phore/thioamide FRET pair
Wissner, Rebecca F.,Wagner, Anne M.,Warner, John B.,Petersson, E. James
, p. 2454 - 2458 (2013)
We have shown that thioamides can be incorporated into proteins through semi-synthesis and used as probes to monitor structural changes. To date, our methods have required the presence of a cysteine at the peptide ligation site, which may not be present in the native peptide sequence. Here, we present a strategy for the semi-synthesis of thioproteins using homocysteine as a ligation point with subsequent masking as methionine, making the ligation 'traceless'. Georg Thieme Verlag Stuttgart, New York.
