15265-26-6

Basic information

• Product Name: PHENOLPHTHALEIN GLUCURONIC ACID
• Synonyms: 1-O-[4-[1-(4-Hydroxyphenyl)-3-oxo-1,3-dihydroisobenzofuran-1-yl]phenyl]-β-D-glucopyranuronic acid;3-[4-(β-D-Glucopyranuronosyloxy)phenyl]-3-(4-hydroxyphenyl)isobenzofuran-1(3H)-one;Phenolphthalein monoglucuronide;Phenolphthalein-monoglucuronide;C016651;Phenolphthalein mono-β-D-glucosiduronic acid;Phenolphthalein-mono-β-glucuronic acid from rabbit urine;Phenolphthalein b-D-glucuronic acid sodium salt
• CAS NO: 15265-26-6
• Molecular Formula: C₂₆H₂₂O₁₀
• Molecular Weight: 494.45
• EINECS: 229-896-3
• Mol File: 15265-26-6.mol
• Article Data: 2

Chemical Properties

• Boiling Point: 804.5°Cat760mmHg
• Flash Point: 276.5°C
• Appearance: /
• Density: 1.584g/cm³
• Vapor Pressure: 2.53E-27mmHg at 25°C
• Storage Temp.: −20°C
• Solubility: H2O: 0.1 g/mL, clear, light yellow
• PKA: 2.77±0.70(Predicted)
• Stability: Hygroscopic
• BRN: 68526
• CAS DataBase Reference: PHENOLPHTHALEIN GLUCURONIC ACID(CAS DataBase Reference)
• NIST Chemistry Reference: PHENOLPHTHALEIN GLUCURONIC ACID(15265-26-6)
• EPA Substance Registry System: PHENOLPHTHALEIN GLUCURONIC ACID(15265-26-6)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 8-10
• Hazardous Substances Data: 15265-26-6(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 15265-26-6 differently. You can refer to the following data:
1. β-glucuronidase test: suitable as substrate, for liver and bacterial enzymes
2. Phenolphthalein β-D-Glucuronide is a commonly used glucuronide conjugate for β-glucuronidase measurements. Highly water-soluble.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

InChI:InChI=1/C26H22O10.Na/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32;/h1-12,19-22,25,27-30H,(H,31,32);/q;+1/p-1/t19-,20-,21+,22-,25+,26?;/m0./s1

Upstream product

• 77-09-8 phenolphthalein 
• 21085-72-3 1-bromo-2,3,4-tri-O-acetyl-α-D-glucuronic acid methyl ester 

Relevant articles and documents

One-pot method for preparing substrate for detecting beta-D-glucuronidase

The invention discloses a one-pot method for preparing a substrate for detecting beta-D-glucuronidase. According to the method, specifically a compound with a structure of a formula (I) and acetobromo-alpha-D-glucuronic acid methyl ester are used as raw materials, the raw materials are subjected to a reaction for 8-24 h in the presence of a polar solvent and a coupling reagent, then an alkaline reagent is added, a reaction is carried out at room temperature for 4-8 h, then an acidic reagent is added to adjust pH to 2-3, and the substrate for detecting beta-D-glucuronidase is prepared through post-treatment. The method uses phenolphthalein with a substituted or un-substituted phenol structure, and acetobromo-alpha-D-glucuronic acid methyl ester as reactants to prepare the substrate for detecting beta-D-glucuronidase, reaction steps and operation are very simple and convenient, the process complexity is reduced, and thereby the expanded production is easy.