153121-60-9Relevant academic research and scientific papers
Zur Totalsynthese von Human-Sekretin
Wuensch, E.,Wendlberger, G.,Goehring, W.,Huebener, G.,Traving, B.
, p. 577 - 586 (2007/10/02)
The synthesis of the heptacosapeptide amide with the primary structure of Human-secretin is described.For this purpose 7 fragments were designed, i.e.H-Gly-Leu-Val-NH2 , Z-Arg(Z2)-Leu-Leu-Gln-OH , Z-Arg(Z2)-Leu-Gln-OH , Z-Arg(Z2)-Glu(OtBu)-Gly-Ala-OH , Z-Arg(Z2)-Leu-OH , Z-Thr(tBu)-Ser(tBu)-Glu(OtBu)-Leu-Ser(tBu)-OH , Adoc-His(Adoc)-Ser(tBu)-Asp(OtBu)-Gly-Thr(tBu)-Phe-OH ; these fragments were consequently assembled to the overall protected total sequence using the Wuensch/Weygand-method with dicyclohexylcarbodiimide.After deprotection by exposure to trifluoroacetic acid in presence of 1,2-ethanediol and water as scavenger, the islated crude product was purified by column chromatography on CM-Sepharose, fast flow.This synthesized Human-secretin showed the full biological activity in comparison to Porcine-secretin. Key words: Gastrointestinal hormone; Human-secretin: synthetic peptide factors.
