153596-97-5Relevant academic research and scientific papers
4-(17-Tetrabenzofluorenylmethyl)-4',4''-Dimethoxytrityl Chloride: A Hydrophobic 5'-Protecting Group for the Separation of Synthetic Oligonucleotides.
Ramage, Robert,Wahl, Franck Olivier
, p. 7133 - 7136 (2007/10/02)
A new highly hydrophobic 5'-hydroxyl protecting group (Tbf-DMTr) has been designed for the purification of synthetic oligonucleotides.Tbf-DMTr-oligonucleotides are strongly retained on RP-HPLC allowing a facile separation from truncated sequences.Subsequently the group can be removed in acidic conditions.The ultra-violet properties of Tbf-DMTr enable easy detection.The synthesis and purification of long oligonucleotides ( >100 mer) is envisaged.Keywords: RP-HPLC; 4-(17-Tetrabenzofluorenylmethyl)-4',4''-Dimethoxytrityl Chloride; Tbf-DMTr; DMTr; Protecting group; Oligonucleotide Synthesis.
