1536470-18-4Relevant academic research and scientific papers
A genetically encoded spin label for electron paramagnetic resonance distance measurements
Schmidt, Moritz J.,Borbas, Julia,Drescher, Malte,Summerer, Daniel
, p. 1238 - 1241 (2014)
We report the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli. This enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chemical labeling step usually required for protein electron paramagnetic resonance (EPR) studies. The amino acid can be introduced at multiple, user-defined sites of a protein and is stable in E. coli even for prolonged expression times. It can report intramolecular distance distributions in proteins by double-electron electron resonance measurements. Moreover, the signal of spin-labeled protein can be selectively detected in cells. This provides elegant new perspectives for in-cell EPR studies of endogenous proteins.
Genetically Encoded Spin Label
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, (2015/07/22)
The invention relates to a non-canonical amino acid of the formula I, namely A - L - X, A is a lysine or a tyrosine, L is a linker or absent, and X is an aminoxyl radical, and if A is lysine, L is bound to the N-epsilon atom of the lysine or, if L is absent, X is bound to the N-epsilon atom of the lysine; and if A is tyrosine, L is bound to the phenolic hydroxyl of the tyrosine or, if L is absent, X is bound to the phenolic hydroxyl of the tyrosine. Moreover, the invention also relates to a method for introducing a spin label into a protein and to a modified pyrrolysyl-tRNA-synthetase.
