1554036-64-4

Basic information

• Product Name: 4,6-O-benzylidene-2-chloro-4-nitrophenyl cellotrioside
• Synonyms: 4,6-O-benzylidene-2-chloro-4-nitrophenyl cellotrioside
• CAS NO: 1554036-64-4
• Molecular Weight: 748.092
• Mol File: 1554036-64-4.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 4,6-O-benzylidene-2-chloro-4-nitrophenyl cellotrioside(CAS DataBase Reference)
• NIST Chemistry Reference: 4,6-O-benzylidene-2-chloro-4-nitrophenyl cellotrioside(1554036-64-4)
• EPA Substance Registry System: 4,6-O-benzylidene-2-chloro-4-nitrophenyl cellotrioside(1554036-64-4)

Safety Data

• Hazardous Substances Data: 1554036-64-4(Hazardous Substances Data)

Upstream product

• 1125-88-8 benzaldehyde dimethyl acetal 
• 161597-23-5 2-chloro-4-nitrophenyl β-cellotrioside 

Downstream Products

• 1453082-10-4 C₁₉H₂₆O₁₁ 

Relevant articles and documents

Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase)

A specific and sensitive substrate for the assay of endo-1,4-β- glucanase (cellulase) has been prepared. The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-β-cellotrioside (BzCNPG3) in the presence of thermostable β-glucosidase. Hydrolysis by exo-acting enzymes such as β-glucosidase and exo-β-glucanase is prevented by the presence of the benzylidene group on the non-reducing end d-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-β-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free d-glucose by the β-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation. This procedure should find widespread applications in biomass enzymology and in the specific assay of endo-1,4-β-glucanase in general.