158962-91-5

Upstream product

• 284676-27-3 4-methylumbelliferyl 2,3,2',3',4'-penta-O-acetyl-β-xylobioside 
• 840502-17-2 4-methylumbelliferyl 2,3,4-tri-O-acetyl-β-D-xylopyranosyl-(1->4)-2,3-di-O-benzoyl-β-D-xylopyranoside 
• 840502-14-9 4-methylumbelliferyl 4-O-chloroacetyl-β-D-xylopyranoside 
• 145370-04-3 4-methylumbelliferyl 2,3-di-O-benzoyl-β-D-xylopyranoside 
• 840502-15-0 4-methylumbelliferyl 2,3-di-O-benzoyl-4-O-chloroacetyl-β-D-xylopyranoside 
• 2771-53-1 ethyl 2,3,4-tri-O-acetyl-1-thio-β-D-xylopyranoside 
• 6734-33-4 4-methylumbellifer-7-yl β-D-xylopyranoside 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 74979-26-3 2,3,2',3',4'-pentaacetyl-α-D-xylobiosyl bromide 
• 183954-79-2 acetyl 2,3,4-tri-O-acetyl-β-D-xylopyranosyl-(1→4)-2,3-di-O-acetyl-D-xylopyranoside 

Downstream Products

• 552-71-6 D-(+)-xylobiose 
• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 

Relevant academic research and scientific papers

Syntheses of 4-methylumbelliferyl-β-D-xylobioside and 5-bromo-3-indolyl-β-D-xylobioside for sensitive detection of xylanase activity on agar plates

4-Methylumbelliferyl-β-D-xylobioside (MU-X2) and 5-bromo-3-indolyl-β-D-xylobioside (BI-X2) were synthesized as substrates for the detection of xylanase activity on agar plates. A family F/10 xylanase from Streptomyces olivaceoviridis E-86 (FXYN) was able to be more sensitively detected than RBB-xylan by using MU-X2 as a substrate. A mutant xylanase E128H/FXYN having only 1/1000 of the activity of FXYN was also able to be detected on the MU-X2 plate but was not detected on the RBB-xylan plate. A family G/11 xylanase from Streptomyces lividans 66 (Xyn B) was not detected on the MU-X2 plate, but it was able to be detected on the RBB-xylan plate, suggesting that the MU-X2 substrate is specific to family F/10 xylanases. However, none of the xylanases were detected effectively by using BI-X2 as a substrate.

An alternative approach for the synthesis of fluorogenic substrates of endo-β-(1→4)-xylanases and some applications

Fluorogenic substrates of endo-β-(1→4)-xylanases (EXs), 4-methylumbelliferyl β-glycosides of xylobiose and xylotriose were synthesized from fully acetylated oligosaccharides using the α-trichloroacetimidate procedure. A commercially available syrup containing xylose and xylo-oligosaccharides was used as the starting material. Both fluorogenic glycosides were found to be suitable substrates for EXs, particularly for sensitive detection of the enzymes in electrophoretic gels and their in situ localization on sections of fruiting bodies of some plants, such as tomato, potato and eggplant, all of the family Solanaceae.

Chemo-enzymatic synthesis of 4-methylumbelliferyl β-(1→4)-D-xylooligosides: New substrates for β-D-xylanase assays

Transglycosylation catalyzed by a β-D-xylosidase from Aspergillus sp. was used to synthesize a set of 4-methylumbelliferyl (MU) β-1→4-D-xylooligosides having the common structure [β-D-Xyl-(1→4)]2-5-β-D-Xyl-MU. MU xylobioside synthesized chemica