159299-28-2

Upstream product

• 55018-48-9 2,3,5-tri-O-benzoyl-1-deoxy-D-ribofuranose 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 491-19-0 1,4-anhydro-D-ribitol 

Downstream Products

• 159299-31-7 2-O-(tert-butyldimethylsilyl)-3-O-[(2-cyanoethoxy)(N,N-diisopropylamino)phosphanyl]-1-deoxy-5-O-(4,4'-dimethoxytrityl)-D-ribofuranose 
• 159299-30-6 2-O-[(tert-butyl)dimethylsilyl]-1-deoxy-5-O-(4,4'-dimethoxytrityl)-D-ribofuranose 

Relevant academic research and scientific papers

Synthesis of fluorobenzene and benzimidazole nucleic-acid analogues and their influence on stability of RNA duplexes

Six different ribonucleoside phosphoramidites with fluorobenzenes or fluorobenzimidazoles as base analogues, one abasic site, and inosine were synthesized and incorporated into oligoribonucleotides. The oligomers were investigated by means of UV and CD spectroscopy to assess the contribution of H-bonding, base stacking, and solvation to the stability of the RNA duplex. CD Spectra show that the incorporation of modified nucleosides does not lead to changes in the structure of RNA. The T(m) differences determined are based on changes in base stacking and solvation. Individual contributions of base stacking and solvation of the modified nucleosides could be determined. In fluorobenzene·fluorobenzimidazole-modified base pairs, a duplex-stabilizing force was found that points to a weak F ··· H H-bond.

S-ANTIGEN TRANSPORT INHIBITING OLIGONUCLEOTIDE POLYMERS AND METHODS

Various embodiments provide STOPS? polymers that are S-antigen transport inhibiting oligonucleotide polymers, processes for making them and methods of using them to treat diseases and conditions. In some embodiments the STOPS? modified oligonucleotides include an at least partially phosphorothioated sequence of alternating A and C units having modifications as described herein. The sequence independent antiviral activity against hepatitis B of embodiments of STOPS? modified oligonucleotides, as determined by HBsAg Secretion Assay, is an EC50 that is less than 100 nM.

Synthesis and biological properties of chemically modified siRNAs bearing 1-deoxy-d-ribofuranose in their 3′-overhang region

To elucidate the role of the sugar moiety in the two natural nucleotides of the 3′-overhang region of small interfering RNA (siRNA), we synthesized siRNAs that incorporated two abasic nucleosides, 1-deoxy-d-ribofuranose (R H). We improved the method for preparing an O-protected abasic nucleoside, 1-deoxy-2,3,5-tri-O-benzoyl-β-d-ribofuranose, via the reductive cleavage of the anomeric position of 1-O-acetyl-2,3,5-tri-O-benzoyl-β-d- ribofuranose. To incorporate RH into oligonucleotides by the standard phosphoramidite solid phase method, RH was converted into its phosphoramidite derivative and the solid support linked to a controlled pore glass resin. Chemically modified RNAs possessing RH at the 3′-overhang region were easily prepared in good yields. siRNAs containing RH showed moderate nuclease-resistance and a desirable knockdown effect.

New structural motifs for hammerhead ribozymes. Catalytic activity of abasic nucleotide substituted ribozymes

The synthesis of 1-deoxy-D-ribofuranose-3-(2-cyanoethyl N,N- diisopropylphosphoramidite) (6) from D-ribose and its incorporation into a hammerhead ribozyme is described.