1595-68-2Relevant academic research and scientific papers
A highly selective FRET-based fluorescent probe for detection of cysteine and homocysteine
Shiu, Hoi-Yan,Chong, Hiu-Chi,Leung, Yun-Chung,Wong, Man-Kin,Che, Chi-Ming
supporting information; scheme or table, p. 3308 - 3313 (2010/06/19)
Fluorescent probe: A turn-on FRET-based fluorescent probe for selective detection of cysteine (Cys) and homocysteine (Hey) under physiological conditions was synthesised (see figure). The probe features excellent selectivity, fast response times and good linearity towards Cys and Hey detection
Electron-deficient alkynes as cleavable reagents for the modification of cysteine-containlng peptides in aqueous medium
Shiu, Hoi-Yan,Chan, Tak-Chung,Ho, Chi-Ming,Liu, Yungen,Wong, Man-Kin,Che, Chi-Ming
supporting information; experimental part, p. 3839 - 3850 (2009/12/31)
An efficient method has been developed for the chemoselective cysteine modification of unprotected peptides and proteins in aqueous media through the formation of a vinyl sulfide linkage by using electron-deficient alkynes, including alkynoic amides, esters and alkynones. The terminal alkynone-modified peptides could be converted back into the unmodified peptides (81 % isolated yield) by adding thiols under mild conditions. The usefulness of this thiol-assisted cleavage of the vinyl sulfide linkage in peptides has been exemplified by the enrichment of a cysteine-containing peptide (71% recovery) from a mixture of cysteine-containing and non-cysteine-containing peptides.
4- AND 5-ALKYNYLOXINDOLES AND 4- AND 5-ALKENYLOXINDOLES
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Page/Page column 11; 21, (2010/02/04)
4- and 5-alkynyloxindoles as well as 4- and 5-alkenyloxindoles having formula (I) and (II), wherein R, R, R, R, R, X and z have the meaning indicated in the specification, inhibit or modulate protein kinases, in particular JNK protein kinases and are useful as anti-inflammatory agents, particularly in the treatment of rheumatoid arthritis.
