16033-31-1

Usage

Uses

Used in Medical Applications:
Neoagarotetraose is used as a potential therapeutic agent for various health conditions due to its bioactive properties, such as anti-obesity, anti-angiogenic, prebiotic, and neuroprotective effects.
Used in Health Food Industry:
Neoagarotetraose is used as a functional ingredient in health foods, leveraging its potential health benefits. However, more research is needed to fully understand its potential and ensure its safety for these applications.
Used in Scientific Research:
Neoagarotetraose is used as a subject of study in various scientific research projects, where its bioactive properties are explored for potential applications in medicine and health food industries.

Relevant academic research and scientific papers

Overexpression and characterization of a novel thermostable β-agarase YM01-3, from marine bacterium Catenovulum agarivorans YM01T

Genome sequencing of Catenovulum agarivorans YM01T reveals 15 open-reading frames (ORFs) encoding various agarases. In this study, extracellular proteins of YM01T were precipitated by ammonium sulfate and separated by one-dimensional gel electrophoresis. The results of in-gel agarase activity assay and mass spectrometry analysis revealed that the protein, YM01-3, was an agarase with the most evident agarolytic activity. Agarase YM01-3, encoded by the YM01-3 gene, consisted of 420 amino acids with a calculated molecular mass of 46.9 kDa and contained a glycoside hydrolase family 16 β-agarase module followed by a RICIN superfamily in the C-terminal region. The YM01-3 gene was cloned and expressed in Escherichia coli. The recombinant agarase, YM01-3, showed optimum activity at pH 6.0 and 60°C and had a Km of 3.78 mg mL-1 for agarose and a Vmax of 1.14 × 104 U mg-1. YM01-3 hydrolyzed the β-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products. Notably, YM01-3 was stable below 50°C and retained 13% activity after incubation at 80°C for 1 h, characteristics much different from other agarases. The present study highlights a thermostable agarase with great potential application value in industrial production.

A simple method of preparing diverse neoagaro-oligosaccharides with β-agarase

In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant β-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13C NMR spectroscopy.

13C-N.M.R.-SPECTROSCOPIC INVESTIGATION OF AGAROSE OLIGOMRS

A complete, unambiguous assignment of all of the 13C-n.m.r.-spectral signals of agarose oligomers produced by enzymic hydrolysis has been achieved.The 1J 13C-H coupling constants are reported, and the chemical shifts and coupling constants of both the agarose polymer and oligomers are compared.