160888-75-5Relevant academic research and scientific papers
Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems
Bier, Claus,Binder, Dennis,Bitzenhofer, Nora Lisa,Drepper, Thomas,Haase, Mona,Hilgers, Fabienne,Hogenkamp, Fabian,Jaeger, Karl-Erich,Ophoven, Vera,Pietruszka, J?rg
, (2021/12/06)
Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically characterized, with the absorption exhibiting a red-shift. Their differing linkage through ether, carbonate, and carbamate bonds revealed that carbonate and carbamate bonds are convenient. Subsequently, those compounds were successfully applied in vivo for controlling gene expression in E. coli via blue light illumination. Furthermore, benzoate-based expression systems were subjected to light control by establishing a novel photocaged salicylic acid derivative. Besides its synthesis and in vitro characterization, we demonstrate the challenging choice of a suitable promoter system for light-controlled gene expression in E. coli. We illustrate various bottlenecks during both photocaged inducer synthesis and in vivo application and possibilities to overcome them. These findings pave the way towards novel caged inducer-dependent systems for wavelength-selective gene expression.
Photochemical gating of intracellular Ca2+ release channels
Ni, Jiahong,Auston, Darryl A.,Freilich, David A.,Muralidharan, Sukumaran,Sobie, Eric A.,Kao, Joseph P. Y.
, p. 5316 - 5317 (2008/02/03)
We have synthesized BiNiX, a caged methylxanthine agonist for the ryanodine receptor (RyR) - the major calcium channel that mediates Ca2+ release from intracellular Ca2+ stores in electrically excitable cells. BiNiX is easily loaded
