1610690-86-2Relevant academic research and scientific papers
Lanthanide-chelating carbohydrate conjugates are useful tools to characterize carbohydrate conformation in solution and sensitive sensors to detect carbohydrate-protein interactions
Canales, ángeles,Mallagaray, álvaro,Berbís, M. álvaro,Navarro-Vázquez, Armando,Domínguez, Gema,Ca?ada, F. Javier,André, Sabine,Gabius, Hans-Joachim,Pérez-Castells, Javier,Jiménez-Barbero, Jesús
, p. 8011 - 8017 (2014)
The increasing interest in the functional versatility of glycan epitopes in cellular glycoconjugates calls for developing sensitive methods to define carbohydrate conformation in solution and to characterize protein-carbohydrate interactions. Measurements of pseudocontact shifts in the presence of a paramagnetic cation can provide such information. In this work, the energetically privileged conformation of a disaccharide (lactose as test case) was experimentally inferred by using a synthetic carbohydrate conjugate bearing a lanthanide binding tag. In addition, the binding of lactose to a biomedically relevant receptor (the human adhesion/growth-regulatory lectin galectin-3) and its consequences in structural terms were defined, using Dy3+, Tb3+, and Tm3+. The described approach, complementing the previously tested protein tagging as a way to exploit paramagnetism, enables to detect binding, even weak interactions, and to characterize in detail topological aspects useful for physiological ligands and mimetics in drug design.
