1616135-85-3Relevant academic research and scientific papers
Water-soluble triarylboron compound for ATP imaging in vivo using analyte-induced finite aggregation
Li, Xiaoyan,Guo, Xudong,Cao, Lixia,Xun, Zhiqing,Wang, Shuangqing,Li, Shayu,Li, Yi,Yang, Guoqiang
, p. 7809 - 7813 (2014)
Adenosine 5′-triphosphate (ATP) is a multifunctional molecule that participates in many important biological processes. Currently, fluorescence indicators for ATP with high performance are in demand. Reported herein is a novel water-soluble triarylboron compound which displays an apparent ATP-dependent fluorescence enhancement when dispersed in water. It can selectively recognize ATP from other bioactive substances in vitro and in vivo. The ATP-induced finite aggregation endows the indicator with appreciable photostability and superior tolerance to environmental electrolytes. This indicator has been successfully applied to the ATP imaging in NIH/3T3 fibroblast cells. The difference in the ATP levels within the membrane and cytosol is clearly visible. Tracking device: The triarylboron compound in the scheme displays an apparent ATP-dependent fluorescence enhancement. The analyte, ATP, induces finite aggregation and endows the indicator with appreciable photostability and superior tolerance to environmental electrolytes, thus leading to its successful application to the monitoring of ATP levels in vitro and in vivo. ATP=adenosine 5′-triphosphate.
ATP (adenosine triphosphate) fluorescent probe based on TPE (tetraphenyl ethylene) as well as preparation method and application of fluorescence probe
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Paragraph 0063; 0064; 0065; 0066, (2018/04/28)
The invention relates to a fluorescence probe shown in general formula (I) and a preparation method of the fluorescence probe. The probe takes TPE (tetraphenyl ethylene) as a fluorescence parent, andwhen the probe is completely dissolved in an aqueous solution, the fluorescence signal is weak; when the probe gathers or forms a solid state, the fluorescence signal is significantly enhanced. The probe has good selectivity for ATP (adenosine triphosphate) and can be used as an ATP fluorescence probe. The probe can be used for detecting in-vitro ATP when prepared in an aqueous solution or a buffer solution. The compound can automatically permeate cell membranes to enter cells without any vectors and can be used for intracellular ATP imaging. The fluorescence probe has the advantages of high specifity, light stability, wide response range and the like.
Water-soluble three arylboron compounds and its preparation method and application
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Paragraph 0058-0061, (2017/04/29)
The invention provides a water-soluble triarylboron compound as well as a preparation method of the triarylboron compound. The triarylboron compound with excellent selectivity to ATP (adenosine triphosphate) can serve as an ATP fluorescent probe, which can be used for detecting in-vitro ATP after being mixed in an aqueous solution or a buffer solution. Without the help of any carrier, the triarylboron compounds can permeate a cell membrane to enter cells and can be use for intracellular ATP imaging. The fluorescent probe has the advantages of efficient specificity, light stability, wide response range and the like.
