162303-66-4 Usage
Description
D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is a chemical compound utilized in biochemical research and analysis. It serves as a substrate for the detection of proteolytic enzyme activity, specifically for the assessment of trypsin and chymotrypsin activity. The P-nitroanilide group is cleaved from the D-VAL-LEU-ARG peptide by proteolytic enzymes, resulting in the release of a yellow color that can be quantified using spectrophotometry. D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is an essential tool in enzymatic assays for studying protease activity and inhibition, thereby aiding in the comprehension of various biological processes.
Uses
Used in Biochemical Research and Analysis:
D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is used as a substrate for the detection of proteolytic enzyme activity, particularly for the determination of trypsin and chymotrypsin activity. It is employed to study the mechanisms of protease activity and inhibition, which is crucial for understanding various biological processes.
Used in Enzymatic Assays:
In enzymatic assays, D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is used as a tool to measure protease activity. The cleavage of the P-nitroanilide group from the peptide by proteolytic enzymes releases a yellow color, which can be quantified spectrophotometrically, providing a means to assess enzyme activity levels.
Used in Pharmaceutical and Biomedical Industries:
D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is used as a reagent in the development and testing of new drugs targeting proteolytic enzymes. Its ability to measure enzyme activity makes it valuable in drug discovery and optimization processes, as well as in the evaluation of potential therapeutic agents that modulate protease activity.
Used in Diagnostic Applications:
In diagnostic applications, D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT can be employed as a diagnostic tool to assess the activity of specific proteolytic enzymes, which may be indicative of certain diseases or conditions. Monitoring enzyme activity can provide insights into disease progression and response to treatment.
Used in Educational Settings:
D-VAL-LEU-ARG P-NITROANILIDE HYDROCHLORIDE SALT is used as an educational tool in teaching laboratories to demonstrate the principles of enzyme kinetics, substrate specificity, and the mechanisms of proteolytic enzyme activity. It provides students with hands-on experience in conducting enzymatic assays and understanding the role of proteases in biological systems.
Check Digit Verification of cas no
The CAS Registry Mumber 162303-66-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,6,2,3,0 and 3 respectively; the second part has 2 digits, 6 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 162303-66:
(8*1)+(7*6)+(6*2)+(5*3)+(4*0)+(3*3)+(2*6)+(1*6)=104
104 % 10 = 4
So 162303-66-4 is a valid CAS Registry Number.