1655-57-8

Usage

Uses

Used in Biochemistry and Molecular Biology Research:
N-(2,4-Dinitrophenyl)-L-leucine is used as a research tool for studying protein structure and function. Its dinitrophenyl group allows for the detection and analysis of amino acids, providing insights into the mechanisms of protein interactions and enzymatic activities.
Used in Enzyme Kinetics Studies:
In enzyme kinetics, DNPL-leucine is utilized as a substrate or inhibitor to investigate the catalytic properties of enzymes. Its chromophoric dinitrophenyl group enables the monitoring of enzyme reactions, facilitating the determination of kinetic parameters such as enzyme activity, substrate specificity, and reaction rates.
Used in Chiral Derivatization for Amino Acid Analysis:
N-(2,4-Dinitrophenyl)-L-leucine is used as a chiral derivatizing reagent in high-performance liquid chromatography (HPLC) for the analysis of amino acids. The derivatization process enhances the separation and detection of enantiomers, allowing for the accurate determination of amino acid concentrations and stereochemistry in various biological samples.

Upstream product

• 99-65-0 meta-dinitrobenzene 
• 328-39-2 LEUCINE 
• 61-90-5 L-leucine 
• 97-00-7 1-chloro-2,4-dinitro-benzene 
• 584-48-5 2,4-dinitrobromobenzene 
• 70-34-8 2,4-Dinitrofluorobenzene 

Relevant academic research and scientific papers

ANTIMICROBIAL PEPTIDES AND METHODS OF USE

Cyclic depsipeptide-class molecules, referred to herein as persephacins (including analogs thereof), having similarities to aureobasidin A, are described. The persephacins have antimicrobial activity, such as antifungal activity against a diverse range of clinically -relevant fungal pathogens, antiprotozoan parasite activity, and antibacterial activity, and can be used for example in treatments of difficult-to-treat ocular fungal infections at lower concentrations than natamycin. The active compounds may be combined with a secondary compound in a composition.

Efficient nucleophilic substitution reaction of aryl halides with amino acids under focused microwave irradiation

The nucleophilic substitution reaction of 2,4-dinitrofluorobenzene with amino acids was complete, under microwave iradiation, within 40 s with yields up to 93%, which are far superior to those obtained under conventional heating. (C) 2000 Elsevier Science Ltd.

Simple and efficient preparation of (R)- and (S)-enantiomers of α-carbon deuterium-labelled α-amino acids

A procedure for the synthesis of (R)- and (S)-enantiomers of α-carbon deuterium-labelled α-amino acids, exemplified for (R)- and (S)-[2-2H1]-Leu is described. Starting from the respective (S)- or (R)-enantiomer or from the racemic mixture of an α-amino acid the selective proton exchange at the α-carbon is carried out by racemization via a Schiff base in monodeuterated acetic acid as solvent which serves as deuterium source. After N-protection the racemic mixture is liquid chromatographically separated into the individual (R)- and (S)-enantiomers on preparative scale employing a chiral anion exchanger based on carbamoylated quinine as chiral selector. After deprotection the enantiomerically pure products can be obtained in good yields.