Tris(hydroxymethyl)chloroacetamidomethane, also known as tris buffer, is a widely used chemical compound in various scientific applications. It is a colorless, crystalline solid with the chemical formula C4H8ClNO4. This buffer is commonly used in biochemistry and molecular biology to maintain a stable pH environment in solutions, particularly in enzyme assays, electrophoresis, and other laboratory procedures. Tris buffer is known for its ability to resist changes in pH over a wide range of temperatures and is often combined with other buffering agents to create a more robust buffer system. Its chemical structure features a central carbon atom bonded to three hydroxymethyl groups and a chloroacetamido group, which contributes to its buffering capacity.
The CAS Registry Mumber 1709-09-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,7,0 and 9 respectively; the second part has 2 digits, 0 and 9 respectively. Calculate Digit Verification of CAS Registry Number 1709-09: (6*1)+(5*7)+(4*0)+(3*9)+(2*0)+(1*9)=77 77 % 10 = 7 So 1709-09-7 is a valid CAS Registry Number.