17682-83-6Relevant articles and documents
N-ACYLATED PHENYLALANINE p-NITROANILIDES: NEW SUBSTRATES FOR CHYMOTRYPSIN
Kasafirek, Evzen,Bartik, Michal
, p. 442 - 451 (2007/10/02)
Chromogenic substrates of the following types were synthesized: X-Phenylalanine p-nitroanilides, where X = acetyl, hydroxyacetyl, methoxyethoxyacetyl, and 3-carboxy-2-propenoyl, (glycyl)n-phenylalanine p-nitroanilides and 3-carboxypropanoyl(glycyl)n-phenylalanine p-nitroanilides, where n= 1,2,3, (glycyl)n-tyrosine p-nitroanilides and 3-carboxypropanoyl(glycyl)n-tyrosine p-nitroanilides, where n= 0,1,2, and also acetylhistidyl-glycyl-glycyl-phenylalanine p-nitroanilide.The konstants Km, kcat and C (i.e. kcat/Km) were estimated for enzymatic splitting by chymotrypsin.The greatest kcat values were found for 3-carboxypropanoyl-glycyl-glycyl-tyrosine p-nitroanilide and 3-carboxypropanoyl-glycyl-glycyl-phenylalanine p-nitroanilide, 40.5 and 16.9, respectively.The smallest Km value, 1.1E-04 (mM) was observed with acetylhistidyl-glycyl-glycyl-phenylalanine p-nitroanilide, which exhibited also the greatest C constant, 115 000 (M-1s-1).