17817-20-8 Usage
Uses
Different sources of media describe the Uses of 17817-20-8 differently. You can refer to the following data:
1. Ultra sensitive fluorogenic substrate for ?-galactosidase, used in live cell and tissue (in vivo and in vitro) assays. This fluorescent substrate reagent could be used for monitoring kinetics of hydrolysis.
2. For detecting Glycosidases and could be used in ELISAs, FDG substrate has also been used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry
3. Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Biochem/physiol Actions
Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.
Check Digit Verification of cas no
The CAS Registry Mumber 17817-20-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,7,8,1 and 7 respectively; the second part has 2 digits, 2 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 17817-20:
(7*1)+(6*7)+(5*8)+(4*1)+(3*7)+(2*2)+(1*0)=118
118 % 10 = 8
So 17817-20-8 is a valid CAS Registry Number.
InChI:InChI=1/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1
