179762-53-9Relevant academic research and scientific papers
Modified Nucleosides for Ribozyme Structure-Activity Studies
Matulic-Adamic, Jasenka,Karpeisky, Alexander M.,Gonzales, Carolyn,Burgin, Alex B.,Usman, Nassim,et al.
, p. S271 - S275 (2007/10/03)
C-Phenyl, C-p-aminophenyl and C-naphthyl as well as pyridine-4(2)-one ribofuranosides were synthesized and site-specifically incorporated into a hammerhead ribozyme using solid phase synthesis.The modified oligonucleotides were used to probe the structural requirements at position 7 for catalytic activity.A pyridine-4-one-base substitution at a single position (N7) within the ribozyme catalytic core increased catalytic rate 10-fold.C-Phenyl, N3-methyluracyl, and p-aminophenyl base substitution at N7 reproducibly increased the catalytic rate twofold.
