180611-60-3Relevant academic research and scientific papers
Polycyclic aromatic DNA-base surrogates: High-affinity binding to an adenine-specific base-flipping DNA methyltransferase
Beuck, Christine,Singh, Ishwar,Bhattacharya, Anupam,Hecker, Walburga,Parmar, Virinder S.,Seitz, Oliver,Weinhold, Elmar
, p. 3958 - 3960 (2003)
Filling the hole is a strategy that confers high-affinity DNA binding to the M·TaqI DNA methyltransferase. Aromatic base surrogates (e.g. pyrene, red in picture) were introduced into DNA by means of organocuprate-mediated C-glycosylations. A new competitive binding assay revealed that DNA with aromatic base surrogates placed opposite to the target base binds to M·TaqI with up to 400-fold-enhanced affinity.
