182005-27-2Relevant academic research and scientific papers
Site-specific cross-linking of proteins to DNA: Via a new bioorthogonal approach employing oxime ligation
Pujari, Suresh S.,Zhang, Yi,Ji, Shaofei,Distefano, Mark D.,Tretyakova, Natalia Y.
supporting information, p. 6296 - 6299 (2018/06/21)
DNA-protein cross-links (DPCs) are super-bulky DNA adducts induced by common chemotherapeutic agents, reactive oxygen species, and aldehydes, and also formed endogenously as part of epigenetic regulation. Despite their presence in most cells and tissues, the biological effects of DPCs are poorly understood due to the difficulty of constructing site-specific DNA-protein conjugates. In the present work, a new approach of conjugating proteins to DNA using oxime ligation was used to generate model DPCs structurally analogous to lesions formed in cells. In our approach, proteins and peptides containing an unnatural oxy-Lys amino acid were cross-linked to DNA strands functionalized with 5-formyl-dC or 7-(2-oxoethyl)-7-deaza-dG residues using oxime ligation. The conjugation reaction was site-specific with respect to both protein and DNA, provided excellent reaction yields, and formed stable DPCs amenable to biological evaluation.
Facile new method for preparation of optically active protected proline
Yamaguchi, Jun-Ichi,Ueki, Masaaki
, p. 621 - 622 (2007/10/03)
Treatment of L-N-protected 2-amino-5-bromopentanoic acid ester, which was prepared from protected L-glutamic acid, with sodium hydride in tetrahydrofuran (THF) proceeded to give the corresponding protected L-proline in high yield. On the other hand, the reaction of 2-aminobutyric acid derivative with sodium hydride gave the 1-aminocyclopropane-1-carboxylic acid derivative.
