18228-17-6 Usage
Uses
Used in Diagnostic Applications:
Naphthol AS-E phosphate is used as a diagnostic tool for detecting and measuring the activity of alkaline phosphatase in biological samples. Its specificity for alkaline phosphatase allows for accurate assessment of enzyme activity, which can be indicative of certain diseases or conditions.
Used in Research Applications:
In research, Naphthol AS-E phosphate is used as a substrate to study the activity of alkaline phosphatase and its role in various biochemical processes. This helps researchers gain insights into enzyme kinetics, cell signaling, and other biological mechanisms.
Used in Enzyme-Linked Immunosorbent Assays (ELISA):
Naphthol AS-E phosphate is used as a substrate in ELISA to measure the presence and activity of alkaline phosphatase in samples. The color change produced upon hydrolysis by alkaline phosphatase allows for the quantification of enzyme activity, which can be used to evaluate the effectiveness of treatments or monitor disease progression.
Used in Pharmaceutical Development:
The specificity of Naphthol AS-E phosphate for alkaline phosphatase makes it a valuable tool in the development of pharmaceuticals targeting this enzyme. It can be used to screen for potential drug candidates and evaluate their efficacy in modulating alkaline phosphatase activity.
Used in Biochemical Education:
Naphthol AS-E phosphate can be used in educational settings to teach students about enzyme kinetics, substrate specificity, and the principles of colorimetric assays. By conducting experiments with NAPHTHOL AS-E PHOSPHATE, students can gain hands-on experience in biochemical analysis and develop a deeper understanding of enzyme function and regulation.
Check Digit Verification of cas no
The CAS Registry Mumber 18228-17-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,8,2,2 and 8 respectively; the second part has 2 digits, 1 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 18228-17:
(7*1)+(6*8)+(5*2)+(4*2)+(3*8)+(2*1)+(1*7)=106
106 % 10 = 6
So 18228-17-6 is a valid CAS Registry Number.
InChI:InChI=1/C17H13ClNO5P/c18-13-5-7-14(8-6-13)19-17(20)15-9-11-3-1-2-4-12(11)10-16(15)24-25(21,22)23/h1-10H,(H,19,20)(H2,21,22,23)
18228-17-6Relevant academic research and scientific papers
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
