BCIP DIPOTASSIUM SALT, also known as 5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt, is an organic compound that serves as a chromogenic substrate for detecting the enzyme alkaline phosphatase in biochemical assays. It is characterized by its stability and solubility in aqueous solutions, making it a reliable reagent for visualizing alkaline phosphatase activity in various biological samples.
185335-30-2
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Basic information
- Product Name: BCIP DIPOTASSIUM SALT
- Synonyms: BCIP;BCIP DIPOTASSIUM SALT;BCIP 2 K;5-BROMO-4-CHLOROINDOL-3-YL PHOSPHATE DIPOTASSIUM SALT;5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, POTASSIUM SALT;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DIPOTASSIUM SALT;X-PHOS 2 K;X-PHOSPHATE POTASSIUM SALT
- CAS NO:185335-30-2
- Molecular Formula: C8H4BrClK2NO4P
- Molecular Weight: 402.65
- EINECS: N/A
- Product Categories: N/A
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: −20°C
- Solubility: N/A
- CAS DataBase Reference: BCIP DIPOTASSIUM SALT(CAS DataBase Reference)
- NIST Chemistry Reference: BCIP DIPOTASSIUM SALT(185335-30-2)
- EPA Substance Registry System: BCIP DIPOTASSIUM SALT(185335-30-2)
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Safety Data
- Hazard Codes: N/A
- Statements: 36
- Safety Statements: 26
- WGK Germany: 1
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 185335-30-2(Hazardous Substances Data)
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185335-30-2 Usage
Uses
Used in Molecular Biology:BCIP DIPOTASSIUM SALT is used as a substrate for detecting alkaline phosphatase activity in molecular biology applications. It produces a blue precipitate upon enzymatic cleavage, allowing researchers to visualize the presence of alkaline phosphatase in DNA, RNA, and protein samples.
Used in Immunohistochemistry:
In immunohistochemistry, BCIP DIPOTASSIUM SALT is used as a chromogenic substrate to detect alkaline phosphatase-tagged antibodies. The blue precipitate generated upon enzymatic cleavage helps visualize the spatial distribution of target antigens in tissue sections, providing valuable information on protein expression patterns and cellular localization.
Used in Diagnostic Assays:
BCIP DIPOTASSIUM SALT is employed as a diagnostic tool in various clinical and research assays to assess alkaline phosphatase levels. The blue precipitate formation serves as a visual indicator of enzyme activity, aiding in the diagnosis and monitoring of conditions associated with altered alkaline phosphatase levels, such as liver and bone disorders.
Used in Educational Purposes:
In educational settings, BCIP DIPOTASSIUM SALT is utilized as a teaching aid to demonstrate enzyme-substrate interactions and enzyme kinetics. The visual blue precipitate formation provides a clear and engaging way for students to understand the principles of enzyme activity and its detection methods.
Check Digit Verification of cas no
The CAS Registry Mumber 185335-30-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,8,5,3,3 and 5 respectively; the second part has 2 digits, 3 and 0 respectively.Calculate Digit Verification of CAS Registry Number 185335-30:
(8*1)+(7*8)+(6*5)+(5*3)+(4*3)+(3*5)+(2*3)+(1*0)=142
142 % 10 = 2
So 185335-30-2 is a valid CAS Registry Number.
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185335-30-2Downstream Products
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BCIP DIPOTASSIUM SALT
