185699-13-2Relevant academic research and scientific papers
Enzymatic Synthesis of Arginine-Containing Peptides - Chromophore Substrates of Metalloproteinases and Carbopeptidases
Yusupova, M. P.,Kotlova, E. K.,Timokhina, E. A.,Stepanov, V. M.
, p. 27 - 32 (2007/10/02)
Subtilisin 72 sorbed on the surface of macroporous glass catalyzes condensation of N-acylated peptide esters with arginine derivatives in organic solvents.The sorbed enzyme can be used repeatedly, which makes it possible to synthesize the chromophore substrates of metalloproteinases and carbopeptidases with the general formula Dnp-Ala-Ala-Xaa-Arg-NH2 (Xaa = Leu, Phe, Val, Ile).In the tetrapeptides, metalloproteinases hydrolyze the Ala-Xaa bond and cleave off Dnp-Ala-Ala-OH, which can be determined spectrophotometrically.The chromophore substrates of B type carboxypeptidases (Dnp-Ala-Ala-Xaa-Arg-OH and Dnp-Ala-Ala-Arg-OH) are obtained by hydrolysis of the corresponding amides with trypsin. Key words: enzymatic synthesis, subtilisin, chromophore substrate, metalloproteinase.
