185971-89-5

Basic information

• Product Name: Fluorescein Alkynylamino-ATP
• Synonyms: Fluorescein Alkynylamino-ATP;N-[6-[[3-[4-AMino-7-[5-O-[hydroxy[[hydroxy(phosphonooxy)phosphinyl]oxy]phosphinyl]-β-D-ribofuranosyl]-7H-pyrrolo[2,3-d]pyriMidin-5-yl]-2-propynyl]aMino]-6-oxohexyl]-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxaMide
• CAS NO: 185971-89-5
• Molecular Formula: C41H41N6O20P3
• Molecular Weight: 1030.713723
• Product Categories: Carbohydrates & Derivatives;Fluorescent Labels & Indicators;Heterocycles;Phosphorylating and Phosphitylating Agents;Carbohydrates & Derivatives, Fluorescent Labels & Indicators, Heterocycles, Phosphorylating and Phosphitylating Agents
• Mol File: 185971-89-5.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• CAS DataBase Reference: Fluorescein Alkynylamino-ATP(CAS DataBase Reference)
• NIST Chemistry Reference: Fluorescein Alkynylamino-ATP(185971-89-5)
• EPA Substance Registry System: Fluorescein Alkynylamino-ATP(185971-89-5)

Safety Data

• Hazardous Substances Data: 185971-89-5(Hazardous Substances Data)

Usage

Description

Fluorescein Alkynylamino-ATP is a fluorescently labeled adenosine triphosphate (ATP) derivative that incorporates a unique alkynylamino group and a fluorescein moiety. This molecule is designed to serve as a versatile tool in various biochemical and molecular biology applications due to its fluorescent properties and ability to act as a substrate for enzymatic reactions.

Uses

Used in Molecular Biology Research:
Fluorescein Alkynylamino-ATP is used as a fluorescent substrate for terminal transferase-catalyzed labeling of DNA sequencing primers. The incorporation of this labeled ATP into the primers allows for the detection and analysis of DNA sequences, which is crucial in various molecular biology techniques, including DNA sequencing, genotyping, and gene expression studies.
Used in Diagnostic Applications:
In the field of diagnostics, Fluorescein Alkynylamino-ATP can be employed as a labeled probe or substrate in assays designed to detect specific nucleic acid sequences or enzymatic activities. The fluorescent signal generated upon binding or reaction can be easily measured and quantified, enabling the sensitive and accurate detection of target molecules or enzymes in various diagnostic tests.
Used in Drug Discovery and Development:
Fluorescein Alkynylamino-ATP can also be utilized in the development of new drugs targeting enzymes that use ATP as a substrate. By monitoring the interaction between the enzyme and the labeled ATP, researchers can gain insights into the enzyme's mechanism of action, identify potential drug targets, and optimize drug candidates for improved efficacy and selectivity.
Used in Cellular Imaging and Analysis:
The fluorescent properties of Fluorescein Alkynylamino-ATP make it an attractive tool for cellular imaging and analysis. It can be used to visualize cellular processes involving ATP, such as energy metabolism, ion transport, and signal transduction. Additionally, the alkynylamino group can be exploited for further functionalization or conjugation to other biomolecules, enabling the development of novel imaging agents or probes for specific cellular targets.