18656-96-7 Usage
Uses
Used in Molecular Biology Research:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is used as a substrate for beta-glucuronidase in molecular biology research for the detection of gene expression. Its ability to produce a blue coloration upon cleavage by the enzyme aids in the visualization and quantification of enzyme activity.
Used in Biochemical Assays:
In biochemical assays, 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is used as a colorimetric indicator for the presence and activity of beta-glucuronidase. The color change provides a means to assess enzyme activity levels, which is crucial for understanding metabolic pathways and enzyme function.
Used in Histochemical Assays:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is used as a histochemical stain to detect beta-glucuronidase activity in tissue sections. This application is valuable for localizing enzyme activity within cells and tissues, offering insights into cellular function and spatial distribution of gene expression.
Used in Gene Regulation Studies:
In the study of gene regulation, 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is used to monitor the activity of genes that encode for beta-glucuronidase. This helps researchers understand the mechanisms of gene regulation and the factors influencing gene expression.
Used in Experimental Protocols for Gene Expression Analysis:
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt is used in various experimental protocols to analyze gene expression. Its solubility in organic solvents allows for its incorporation into a wide range of assays, facilitating the study of gene regulation and expression under different conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 18656-96-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,8,6,5 and 6 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 18656-96:
(7*1)+(6*8)+(5*6)+(4*5)+(3*6)+(2*9)+(1*6)=147
147 % 10 = 7
So 18656-96-7 is a valid CAS Registry Number.
InChI:InChI=1/C18H21BrClNO7.C6H13N/c1-15(24)13(14(22)23)28-18(4,17(3,26)16(15,2)25)27-10-7-21-9-6-5-8(19)12(20)11(9)10;7-6-4-2-1-3-5-6/h5-7,13,21,24-26H,1-4H3,(H,22,23);6H,1-5,7H2/t13?,15-,16u,17u,18-;/m1./s1
