188944-67-4Relevant academic research and scientific papers
A turn-on fluorescent and colorimetric chemodosimeter for selective detection of Au3+ ions in solution and in live cells: Via Au3+-induced hydrolysis of a rhodamine-derived Schiff base
Datta, Pallab,Ghosh, Aritri,Mandal, Debasish,Manna, Saikat Kumar,Mondal, Sanchita,Mukhopadhyay, Subrata,Pathak, Sudipta
, p. 7954 - 7961 (2020)
A chromogenic and off-on fluorogenic chemodosimeter (L) based on a naphthalene-rhodamine B derivative was designed, synthesized and characterized for the selective and sensitive detection of Au3+ ions in mixed acetonitrile aqueous medium in the presence of other competitive analytes. Probe L displayed a 696-fold turn-on fluorescence signal enhancement toward Au3+ ions with the detection limit of Au3+ of about 1.51 μM. In the presence of Au3+ ions, probe L also exhibited a visible color change from colorless to pink that could be easily identified by the naked eye. This colorimetric and fluorometric changes were mainly due to the Au3+ ion-triggered imine bond (CN) hydrolysis sensing mechanism. Moreover, theoretical calculations were performed to explain the experimental results. In addition to these features, the off-on probe was successfully used to monitor intracellular Au3+ ions in living MC3T3 cells through fluorescence imaging.
Multifunctional hydroxyapatite nanoparticle-based affinity adsorbent with sensing and fluorescence imaging capacity
Yao, Shasha,Zhang, Yu,Zhang, Junli,Zhang, Xu,Li, Binjie,Zhao, Yanbao
, p. 7988 - 7995 (2014)
Nickel hydroxide/hydroxyapatite (Ni(OH)2/HAP) nanoparticles (NPs) coated with rhodamine B hydrazide (RBH) were successfully synthesized in three steps. First, Ni(OH)2/HAP-COOH NPs were synthesized by a facile hydrothermal route in the presence of citric acid. Second, rhodamine B was reacted with hydrazine hydrate to obtain rhodamine B hydrazide (RBH). Finally, RBH was conjugated on the surface of Ni(OH)2/HAP-COOH NPs to form Ni(OH)2/HAP-RBH. As affinity adsorbents, these NPs can directly purify histidine-tagged (His-tagged) proteins from a mixture of lysed cells, and exhibit high protein adsorption capacity with a maximum value of 115 mg g-1. A prominent fluorescence enhancement at 578 nm was observed in the presence of Pt2+, accompanied by a change in absorption spectrum. These NPs display excellent selectivity and sensitive recognition of Pt2+ in comparison with other metal ions and anions with a detection limit of 0.03 μM. These NPs exhibit low cytotoxicity and can be used as a probe for Pt2+ and living cell imaging. This journal is
Fluorescence labelled probe as well as preparation method and application thereof in protein labeling
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Paragraph 0030, (2016/12/12)
The invention discloses a fluorescence labelled probe as well as a preparation method and application thereof in protein labeling. An organic synthesis method is adopted, and an oxadiazole heterocycle with fluorescent properties and multiple heteroatoms is directly introduced to the bottom ring of a rhodamine B skeleton structure. The introduced oxadiazole heterocycle serves as a second fluorophore for expanding the spectroscopic properties of rhodamine fluorescence probes; and by introducing multiple heteroatoms, the sites forming hydrogen bonds are increased, and the action with amino acids in the protein is promoted. In a compound RH-MCl, one side of the oxadiazole cycle is substituted by rhodamine B, and the other side is substituted by chloromethyl. The invention also provides a method for gel electrophoresis fluorescence labeling of protein using the fluorescence probe. The compound RH-MCl has good water solubility, and the fluorescence labeling method for protein has the advantages of easiness, short time, high sensitivity, good reproducibility, good labeling background, good compatibility, safety in use, low cost and the like.
