188944-67-4

Basic information

• Product Name: N-(Rhodamine-B) lactam-hydrazine
• Synonyms: N-(Rhodamine-B) lactam-hydrazine
• CAS NO: 188944-67-4
• Molecular Weight: 493.049
• Mol File: 188944-67-4.mol
• Article Data: 3

Chemical Properties

• CAS DataBase Reference: N-(Rhodamine-B) lactam-hydrazine(CAS DataBase Reference)
• NIST Chemistry Reference: N-(Rhodamine-B) lactam-hydrazine(188944-67-4)
• EPA Substance Registry System: N-(Rhodamine-B) lactam-hydrazine(188944-67-4)

Safety Data

• Hazardous Substances Data: 188944-67-4(Hazardous Substances Data)

Upstream product

• 81-88-9 rhodamine B 
• 74317-53-6 rhodamine B hydrazide 

Downstream Products

• 885481-00-5 C₃₅H₃₇N₅O₂S 
• 1033581-68-8 C₃₆H₃₆N₆O₂S₂ 

Relevant articles and documents

A turn-on fluorescent and colorimetric chemodosimeter for selective detection of Au3+ ions in solution and in live cells: Via Au3+-induced hydrolysis of a rhodamine-derived Schiff base

A chromogenic and off-on fluorogenic chemodosimeter (L) based on a naphthalene-rhodamine B derivative was designed, synthesized and characterized for the selective and sensitive detection of Au3+ ions in mixed acetonitrile aqueous medium in the presence of other competitive analytes. Probe L displayed a 696-fold turn-on fluorescence signal enhancement toward Au3+ ions with the detection limit of Au3+ of about 1.51 μM. In the presence of Au3+ ions, probe L also exhibited a visible color change from colorless to pink that could be easily identified by the naked eye. This colorimetric and fluorometric changes were mainly due to the Au3+ ion-triggered imine bond (CN) hydrolysis sensing mechanism. Moreover, theoretical calculations were performed to explain the experimental results. In addition to these features, the off-on probe was successfully used to monitor intracellular Au3+ ions in living MC3T3 cells through fluorescence imaging.

Fluorescence labelled probe as well as preparation method and application thereof in protein labeling

The invention discloses a fluorescence labelled probe as well as a preparation method and application thereof in protein labeling. An organic synthesis method is adopted, and an oxadiazole heterocycle with fluorescent properties and multiple heteroatoms is directly introduced to the bottom ring of a rhodamine B skeleton structure. The introduced oxadiazole heterocycle serves as a second fluorophore for expanding the spectroscopic properties of rhodamine fluorescence probes; and by introducing multiple heteroatoms, the sites forming hydrogen bonds are increased, and the action with amino acids in the protein is promoted. In a compound RH-MCl, one side of the oxadiazole cycle is substituted by rhodamine B, and the other side is substituted by chloromethyl. The invention also provides a method for gel electrophoresis fluorescence labeling of protein using the fluorescence probe. The compound RH-MCl has good water solubility, and the fluorescence labeling method for protein has the advantages of easiness, short time, high sensitivity, good reproducibility, good labeling background, good compatibility, safety in use, low cost and the like.