190834-10-7Relevant academic research and scientific papers
Synthesis of oligonucleotides containing 3'-alkyl amines using isobutyryl protected deoxyadenosine phosphoramidite
McMinn, Dustin L.,Greenberg, Marc M.
, p. 3123 - 3126 (1997)
Oligonucleotides were synthesized using the N-isobutyryldeoxyadenosine protected β-cyanoethyl phosphoramidite, 9. The oligonucleotides that were synthesized using 9 can be deprotected using concentrated NH4OH at 55°C in 2 h. No depurination was detected via anion exchange HPLC. The N-isobutyryl protected deoxyadenosine did not undergo transamidation with nucleobase and phosphate protected oligonucleotides containing primary alkyl amines, and will be useful in the synthesis of bioconjugates using protected biopolymers.
A new and convenient approach for the preparation of β-cyanoethyl protected trinucleotide phosphoramidites
Janczyk, Matthaeus,Appel, Bettina,Springstubbe, Danilo,Fritz, Hans-Joachim,Mueller, Sabine
, p. 1510 - 1513 (2012/03/22)
Herein we report a convenient approach for the preparation of fully protected trinucleotide synthons to be used for the synthesis of gene libraries. The trinucleotide synthons bear β-cyanoethyl groups at the phosphate residues, and thus can be used in standard oligonucleotide synthesis without additional steps for deprotection and work-up.
