192203-23-9Relevant academic research and scientific papers
Fluorescent molecular probes I. The synthesis and biological properties of an ELF β-glucuronidase substrate that yields fluorescent precipitates at the enzymatic activity sites
Diwu, Zhenjun,Lu, Yixin,Upson, Rosalyn H.,Zhou, Mingjie,Klaubert, Dieter H.,Haugland, Richard P.
, p. 7159 - 7164 (2007/10/03)
A novel quinazolinone-based fluorogenic β-glucuronidase substrate has been synthesized and evaluated. In this protocol, a partially protected salicyl glucuronide is oxidatively condensed with anthranilamide to afford the required quinazolinonyl glucuronic acid methyl ester that is readily deprotected to yield the desired glucuronide, known as ELF-97 glucuronide. The glucuronide is a sensitive fluorogenic β-glucuronidase substrate that yields a bright yellow-green fluorescent precipitate upon enzymatic reaction.
