195247-46-2Relevant academic research and scientific papers
Solid-phase synthesis of branched RNA and branched DNA/RNA chimeras
Grotli, Morten,Eritja, Ramon,Sproat, Brian
, p. 11317 - 11346 (2007/10/03)
An effective method for synthesising branched oligonucleotides on solid phase in the 5' to 3' direction has been developed. Special branch-point monomers enable the synthesis of branched oligonucleotides which can have sequences of different length and base composition attached to the 2'- and 3'-hydroxyl groups of the branch point ribonucleoside. The branched oligonucleotides are assembled on commercial DNA synthesisers, the crude products are readily purified by reversed phase HPLC and the fully deprotected products are conveniently analysed by mass spectrometry.
A Universal Solid-phase Synthesis of Branched Oligoribonucleotides
Groetli, Morten,Sproat, Brian S.
, p. 495 - 498 (2007/10/02)
A novel branch-point adenosine monomer is synthesized which enables the routine solid-phase synthesis of branched oligoribonucleotides in the 5' to 3' direction, such that the sequences at the 2' and 3' positions of the branch-point adenosine can be of di
