19963-02-1

Basic information

• Product Name: 2-Butenoic acid, 4-[[4-(6-methyl-2-benzothiazolyl)phenyl]amino]-4-oxo-, (Z)-
• CAS NO: 19963-02-1
• Molecular Formula: C18H14N2O3S
• Molecular Weight: 338.387
• Mol File: 19963-02-1.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 2-Butenoic acid, 4-[[4-(6-methyl-2-benzothiazolyl)phenyl]amino]-4-oxo-, (Z)-(CAS DataBase Reference)
• NIST Chemistry Reference: 2-Butenoic acid, 4-[[4-(6-methyl-2-benzothiazolyl)phenyl]amino]-4-oxo-, (Z)-(19963-02-1)
• EPA Substance Registry System: 2-Butenoic acid, 4-[[4-(6-methyl-2-benzothiazolyl)phenyl]amino]-4-oxo-, (Z)-(19963-02-1)

Safety Data

• Hazardous Substances Data: 19963-02-1(Hazardous Substances Data)

Upstream product

• 108-31-6 maleic anhydride 
• 92-36-4 2-(4-aminophenyl)-6-methyl-1,3-benzothiazole 

Downstream Products

• 19735-68-3 2-(4-N-maleimidophenyl)-6-methylbenzothiazole 

Relevant articles and documents

Determination of aliphatic thiols by fluorometric high-performance liquid chromatography after precolumn derivatization with 2-(4-N-maleimidophenyl)-6-methylbenzothiazole

A sensitive, fluorometric high-performance liquid chromatographic method for the detection of aliphatic thiols, following pre-column derivatization with 2-(4-N-maleimidophenyl)-6-methylbenzothiazole, has been developed. The N-maleimide, the acid and the methyl ester derivatives of the commercially available 2-(4-aminophenyl)-6-methylbenzothiazole were synthesized and found to be equally effective for the precolumn derivatization procedure. The resulting fluorescentic derivatives of aliphatic thiols were separated on a reversed-phase column (Ultrasphere-ODS) using 0.1% hexane-sulfonic acid in 10 mM potassium hydrogen phosphate:acetonitrile (65:35) as a mobile phase and were detected fluorometrically (excitation 320 nm; emission 405 nm). The method is highly sensitive (femtomole range) and is easily applied for determination of SH-containing drugs and endogenous thiols in biological samples.