20325-40-0 Usage
Description
o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid , and glucose. It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites. This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.
Chemical Properties
Off-white or grey tablets
Uses
Different sources of media describe the Uses of 20325-40-0 differently. You can refer to the following data:
1. o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.
2. It is suitable for use in ELISA procedures. In neurohistochemistry o-Dianisidine dihydrochloride has demonstrated perikaryal labeling as well as retrograde and anterograde transport to the primary olfactory cortex. It has also been used for a paper based-micro fluidic device to semi-quantitatively measure lactate, uric acid, and glucose. 3,3'-Dimethoxybenzidine is used principally as an intermediate in the production of commercial bisazobiphenyl dyes for coloring textiles, paper, plastic, rubber, and leather.
General Description
Off-white powder.
Air & Water Reactions
Water soluble.
Reactivity Profile
3,3'-Dimethoxybenzidine dihydrochloride is an ether substituted acidic organic salt. Reacts weakly as a acid to neutralize bases. May react with strong oxidizing agents or strong reducing agents.
Fire Hazard
Flash point data are not available for 3,3'-Dimethoxybenzidine dihydrochloride, but 3,3'-Dimethoxybenzidine dihydrochloride is probably combustible.
Safety Profile
Confirmed carcinogen
with experimental carcinogenic and
tumorigenic data. Experimental
reproductive data. Mutation data reported.When heated to decomposition it emits very
toxic fumes of NO, and HCl.
Check Digit Verification of cas no
The CAS Registry Mumber 20325-40-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,0,3,2 and 5 respectively; the second part has 2 digits, 4 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 20325-40:
(7*2)+(6*0)+(5*3)+(4*2)+(3*5)+(2*4)+(1*0)=60
60 % 10 = 0
So 20325-40-0 is a valid CAS Registry Number.