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3,3'-Dimethoxybenzidine dihydrochloride, also known as o-Dianisidine dihydrochloride, is an off-white or grey powder that serves as a colorimetric peroxidase substrate. It is initially colorless but turns violet upon oxidization, which can be spectrophotometrically measured at a wavelength of 405 nm. 3,3'-Dimethoxybenzidine dihydrochloride is used in various applications due to its unique chemical properties.

20325-40-0

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20325-40-0 Usage

Uses

Used in Glucose Oxidase (GO) Reagent:
3,3'-Dimethoxybenzidine dihydrochloride is used as a component in the glucose oxidase (GO) reagent, which is utilized for measuring the glucose content in samples. Its role in this application is to provide a colorimetric indication of glucose levels, facilitating accurate and reliable measurements.
Used in Enzyme-Linked Immunosorbent Assay (ELISA) Procedures:
3,3'-Dimethoxybenzidine dihydrochloride is also suitable for use in ELISA procedures, where it serves as a colorimetric substrate to detect and quantify specific antibodies or antigens. Its color change upon oxidization allows for easy visualization and quantification of the target molecules in the assay.
Used in Neurohistochemistry:
In the field of neurohistochemistry, 3,3'-Dimethoxybenzidine dihydrochloride has demonstrated its utility in perikaryal labeling, as well as in retrograde and anterograde transport to the primary olfactory cortex. This makes it a valuable tool for studying neural pathways and the transport of molecules within the nervous system.
Used in Paper-based Microfluidic Devices:
3,3'-Dimethoxybenzidine dihydrochloride has been employed in the development of paper-based microfluidic devices for the semi-quantitative measurement of lactate, uric acid, and glucose. Its colorimetric properties make it an ideal choice for these devices, which can be used for point-of-care diagnostics and monitoring of various biomarkers.
Used in Textile, Paper, Plastic, Rubber, and Leather Industries:
3,3'-Dimethoxybenzidine is primarily used as an intermediate in the production of commercial bisazobiphenyl dyes, which are used for coloring textiles, paper, plastic, rubber, and leather. Its ability to produce vibrant and long-lasting colors makes it a valuable component in the manufacturing of these products.
Used as a Reagent for Detection of Various Metals, Thiocyanates, and Nitrites:
Due to its chemical properties, 3,3'-Dimethoxybenzidine dihydrochloride is also used as a reagent for the detection of various metals, thiocyanates, and nitrites. Its colorimetric response to oxidization allows for the identification and quantification of these substances in various samples.

Air & Water Reactions

Water soluble.

Reactivity Profile

3,3'-Dimethoxybenzidine dihydrochloride is an ether substituted acidic organic salt. Reacts weakly as a acid to neutralize bases. May react with strong oxidizing agents or strong reducing agents.

Fire Hazard

Flash point data are not available for 3,3'-Dimethoxybenzidine dihydrochloride, but 3,3'-Dimethoxybenzidine dihydrochloride is probably combustible.

Safety Profile

Confirmed carcinogen with experimental carcinogenic and tumorigenic data. Experimental reproductive data. Mutation data reported.When heated to decomposition it emits very toxic fumes of NO, and HCl.

Check Digit Verification of cas no

The CAS Registry Mumber 20325-40-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,0,3,2 and 5 respectively; the second part has 2 digits, 4 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 20325-40:
(7*2)+(6*0)+(5*3)+(4*2)+(3*5)+(2*4)+(1*0)=60
60 % 10 = 0
So 20325-40-0 is a valid CAS Registry Number.

20325-40-0 Well-known Company Product Price

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  • (Code)Product description
  • CAS number
  • Packaging
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  • Detail
  • TCI America

  • (D3893)  o-Dianisidine Dihydrochloride [for Biochemical Research]  >95.0%(T)

  • 20325-40-0

  • 1g

  • 310.00CNY

  • Detail
  • TCI America

  • (D3893)  o-Dianisidine Dihydrochloride [for Biochemical Research]  >95.0%(T)

  • 20325-40-0

  • 5g

  • 750.00CNY

  • Detail
  • Alfa Aesar

  • (A17175)  o-Dianisidine dihydrochloride, 99%   

  • 20325-40-0

  • 1g

  • 274.0CNY

  • Detail
  • Alfa Aesar

  • (A17175)  o-Dianisidine dihydrochloride, 99%   

  • 20325-40-0

  • 5g

  • 1103.0CNY

  • Detail

20325-40-0SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 11, 2017

Revision Date: Aug 11, 2017

1.Identification

1.1 GHS Product identifier

Product name 3,3'-Dimethoxybenzidine dihydrochloride

1.2 Other means of identification

Product number -
Other names Fast Blue B

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:20325-40-0 SDS

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